ABSTRACT
Objective To investigate the expression of p53,VEGF,E-cad,CK20 and its clinical meanings in bladder transitional cell carcinoma. Methods The specimen microarrays and immunohistochemistry were applied to detect selected cancer-related protein expression in 45 cases of bladder TCC and 8 cases of normal control. Results Abnormal expression of p53,E-cad and CK20 were detected 100.0%,55.6%,55.6% respectively in bladder TCC group,which were expressed normally in control group with significant differences.However,there was no VEGF expression differences between those 2 groups (87.5% v.86.7%).Abnormal expression intensity of p53 and expression rate of E-cad were closely related to the pathological features.CK20 expression was co-related positively with disease staging rather than grading.VEGF played less role in co-relating with pathological features. Conclusions p53,E-cad and CK20 play an important roles in the development of bladder TCC,and VEGF plays less.Microarrays is suggested to detect a large scale of specimen for multiple proteins,it is effective.
ABSTRACT
Objective To study the relationship between vascular endothelial growth factor (VEGF) and occurrence and invasiveness of bladder cancer. Methods The concentration of VEGF in urine was examined by ELISA in 36 cases of transitional cell carcinoma of bladder. Results The concentration of VEGF was low (3.73?2.63 pg/ml) in controls whereas it has been high in bladder carcinoma (210.97?154.68 pg/ml ), P